Identification of genetic relationships and subspecies signatures in Xylella fastidiosa
نویسندگان
چکیده
منابع مشابه
Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques
In the never ending struggle against plant pathogenic bacteria, a major goal is the early identification and classification of infecting microorganisms. Xylella fastidiosa, a Gram-negative bacterium belonging to the family Xanthmonadaceae, is no exception as this pathogen showed a broad range of vectors and host plants, many of which may carry the pathogen for a long time without showing any sy...
متن کاملNatural Competence Rates Are Variable Among Xylella fastidiosa Strains and Homologous Recombination Occurs In Vitro Between Subspecies fastidiosa and multiplex.
Xylella fastidiosa, an etiological agent of emerging crop diseases around the world, is naturally competent for the uptake of DNA from the environment that is incorporated into its genome by homologous recombination. Homologous recombination between subspecies of X. fastidiosa was inferred by in silico studies and was hypothesized to cause disease emergence. However, no experimental data are av...
متن کاملXylella fastidiosa subspecies: X. fastidiosa subsp piercei, subsp. nov., X. fastidiosa subsp. multiplex subsp. nov., and X. fastidiosa subsp. pauca subsp. nov
Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple taxons. To determine the taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S–23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions...
متن کاملA Conjugative 38 kB Plasmid Is Present in Multiple Subspecies of Xylella fastidiosa
A ≈ 38kB plasmid (pXF-RIV5) was present in the Riv5 strain of Xylella fastidiosa subsp. multiplex isolated from ornamental plum in southern California. The complete nucleotide sequence of pXF-RIV5 is almost identical to that of pXFAS01 from X. fastidiosa subsp. fastidiosa strain M23; the two plasmids vary at only 6 nucleotide positions. BLAST searches and phylogenetic analyses indicate pXF-RIV5...
متن کاملDEVELOPMENT OF QRT-PCR PROTOCOLS FOR RAPID XYLELLA FASTIDIOSA SUBSPECIES DIAGNOSTICS Principal Investigator:
Xylella fastidiosa (Xf) is a plant pathogenic bacterium that is transmitted between hosts by the glassy-winged sharpshooter (GWSS; Homalodisca vitripennis). Multiple subspecies of Xf occur and are host specific. Xf subsp. fastidiosa is the causal agent of Pierce’s disease (PD). Xf subsp. multiplex and Xf subsp. sandyi are commonly found in North America but do not cause PD. Rapid diagnostics to...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2019
ISSN: 1471-2164
DOI: 10.1186/s12864-019-5565-9